Ebla Private University

lecturer

Mr. Mudar Sadiq

Master's degree in Pharmacy, specializing in Food Control, from the University of Aleppo, 2014

Contact

Modarsadek@alepuniv.edu.sy

Experiences:

Lecturer at the Faculty of Pharmacy, University of Aleppo: 2002–2026
Lecturer at the Ebla Private University, Faculty of Pharmacy: 2015–2026

Researches:

A sustainable supramolecular deep eutectic solvent system for dispersive liquid–liquid microextraction followed by HPLC for the determination of melamine in milk samples

This work presents the development of an innovative green supramolecular deep eutectic solvent (SUPRADES)-based method for the extraction and quantification of melamine in milk. An extraction solvent was prepared using thymol and nonanoic acid, while a disperser solvent was prepared using choline chloride and ethylene glycol doped with β-cyclodextrin. The method showed good linearity (100–900 ng/mL, R² = 0.998), with an LOD of 27 ng/mL and LOQ of 81 ng/mL. Recovery values ranged from 92–107% with RSD < 5%, and the method achieved a greenness score of 79 according to the ComplexMoGAPI metric. The technique was successfully applied to determine melamine in real milk samples. - Results in Chemistry

10 - January - 2026

Benchmark dose modeling of continuous renal biomarkers for melamine nephrotoxicity in rats to derive a health-protective tolerable daily intake

This study aimed to derive a health-protective tolerable daily intake (TDI) for melamine by applying benchmark dose (BMD) modeling to continuous renal biomarkers in rats. Male Wistar rats were exposed to increasing doses of melamine for 28 days, and dose-response relationships for renal biomarkers such as urea and creatinine were modeled using BMD analysis. The results showed a composite BMDL of 6.815 mg/kg bw/day, which, after applying a 1000-fold uncertainty factor, yielded a TDI of 0.0068 mg/kg bw/day—10- to 30-fold lower than current FDA and EFSA/WHO values. This work provides an advanced scientific framework for reassessing melamine risk and strengthening public health protection standards. - Journal of Hazardous Materials Advances

6 - January - 2026

Assessment of Early Nephrotoxicity of Low-Dose Melamine in Wistar Albino Rats Using Conventional and Novel Biomarkers

This study aimed to assess the renal toxicity of low-dose melamine (8 and 16 mg/kg bw/day) in male Wistar Albino rats over multiple exposure periods. Conventional markers (creatinine and urea) showed no significant changes, except for a significant decrease in estimated glomerular filtration rate (eGFR) in the higher dose group after 4 weeks. Importantly, early biomarkers (NGAL, RBP, and α1-M) revealed dose- and duration-dependent renal injury, with clear changes appearing as early as week three at the higher dose. These findings highlight the sensitivity of early biomarkers in detecting renal injury at low melamine doses, underscoring the need to reconsider safe exposure limits and incorporate these markers into toxicity assessment protocols. - Research Journal of Aleppo University

2 - November - 2025

Assessment of Early Nephrotoxicity of Low-Dose Melamine in Wistar Albino Rats Using Conventional and Novel Biomarkers

2 - November - 2025

Melamine Monitoring in Milk by Reversed-Phase High-Performance Liquid Chromatography Technique

A rapid, selective, and sensitive reversed-phase high-performance liquid chromatography (HPLC) method was developed to determine melamine in milk. The mobile phase was methanol:phosphate buffer at pH = 3 (20:80 v/v). An SGE ProteCol-GP125-C18 (4.6 x 250 mm, 5 µm) column was used to perform the separation, and the detector was a photodiode array at a wavelength of 236 nm.. The parameters such as mobile phase ratios, pH values, and buffer concentrations were tested. The method demonstrated the validation and ability to separate melamine from with high accuracy in less than 5 min, the retention time of melamine was 4.1 min. The method exhibited an acceptable linearity within the concentration range of (100-1000 ng/mL) with a correlation coefficient r2 = 0.999. Under optimum conditions, the limit of detection (LOD) and limit of quantification (LOQ) were 22.65ng/mL and 68.64ng/mL, respectively, with good precisions. The developed method was successfully applied to liquid and powdered milk and infant formula samples, the melamine peak was completely separate from the milk components, and the recovery of melamine was within 87.26- 100.40% in spiked milk containing 250 and 500 ng/mL, with RSD% of 1.34-3.71%. - Aleppo University Research Journal: Medical Science Series

16 - July - 2025

The effect of phytic acid on rate of starch digestibility in Vicia faba and white wheat bread in vitro.

The effect of the phytic acid on starch digestibility was studied in vitro. Starch digestibility was found to correlate negatively with the phytic acid content of the food tested. When sodium phytate was added (equivalent to 2% of phytic acid based on the starch portion), it was noticed that the rate of Vicia faba starch digestion by human saliva and at human body temperature (37°C) was decreased by 13%, while the rate of digestion of white wheat bread decreased by 20%. Furthermore, it was observed that mixing phytic acid with human saliva before adding the former to starch and following a pH adjustment reduced the rate of starch digestion by 6% when compared to mixing phytic acid with starch first and then adding human saliva to it.. - International Journal of Pharmaceutical Sciences and Research (IJPSR)

5 - February - 2014